Abstract—The antimicrobial photodynamic activity of protoporphyrine IX (PPIX) in conjunction with red light - emitting diode (LED) and xenon lamp was carried out against Staphylococcus aureus (S.aureus). At 30min illumination using the red LED and 30μM of PPIX 99.8% reduction of S.aureus survival was achieved. Based on optofluorometric study, it is further observed that the measurement of steady-state native fluorescence of amino acid, tryptophan provided a method to monitor the Antimicrobial photodynamic therapy (APDT) efficiency by optical means.

Index Terms—Antimicrobial photodynamic therapy, red LED, protoporphyrine IX.

Fathi Awad, P. Aruna, and S.Ganesan are with the Department of Medical Physics, Anna University, Chennai 600025, India (e-mail: sganesan@annuniv.edu, Tel:+914422358685; fax: +914422358700).
C.Ramprasath and N.Mathivanan are with the Centre for Advanced Studies in Botany, University of Madras, Chennai 600005.

Cite: Fathi Awad, C. Ramprasath, N. Mathivanan, P. Aruna, and S. Ganesan, "Evaluation of Protoporphyrine IX-Light Emitting Diode Based Antimicrobial Photodynamic Therapy against Staphylococcus Aureus," International Journal of Applied Physics and Mathematics  vol. 2, no. 3, pp.  218-220, 2012.